Official Journal of the European Communities, 143, 26Ĭhen D, Delmas JM, Hurtaud-Pessel D, Verdon E (2020) Development of a multi-class method to determine nitroimidazoles, nitrofurans, pharmacologically active dyes and chloramphenicol in aquaculture products by liquid chromatography-tandem mass spectrometry. Official Journal of the European Communities, L71, 17–18Ĭommission Regulation (EC) 1995/1442/EC (1995). The developed method was successfully applied in 100 aquatic products for NFs metabolites detection and the results were further confirmed by the national standard GB/T 21311-2007 method.Ĭommission Decision (EC) 2003/181/EC (2003). Recovery rates were ranged from 90% to 114% for fortified samples at levels of 1.0 μg kg −1, 5.0 μg kg −1 and 10 μg kg −1 with relative standard deviation values ranging from 1.6% to 5.8%. The linearity range for the four analytes were 0.25–50 μg kg −1 with good determination coefficients ( R 2 > 0.9982). 2-CBA was employed as derivatization reagent in this study, which greatly reduced the derivatization time. The experimental operation consisted of an acid-catalyzed release of protein-bound metabolites, followed by derivatization with 2-chlorobenzaldehyde (2-CBA) and then extraction with ethyl acetate before analysis by positive electrospray ionisation (ESI +) with multiple reaction monitoring (MRM) type. A rapid quantization of nitrofuran metabolites residues in aquatic products by ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was developed in this study.

Nitrofurans, a kind of banned antibiotics for their serious toxic side effects, have often been found in aquatic products in the past and are still found nowadays which were a severe threat for human health.

Mmass of water